Download 8th International Conference on Cell & Stem Cell Engineering by M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El PDF

By M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El Haj, Dan Bader (eds.)

ISBN-10: 364219043X

ISBN-13: 9783642190438

This quantity offers chosen peer-reviewed papers of the eighth foreign convention on mobilephone & Stem phone Engineering (ICCM) 2010 in Dublin. The contributions are written by way of major scientists in phone and Stem cellphone Engineering and the subjects of the papers comprise:
Computational mobilephone Mechanics
Experimental suggestions in mobile Mechanics
Molecular and telephone Imaging
Cell Matrix Interactions
Mechanotransduction and telephone mechanics
Cell sensing
Cell processing
Artificial cells
Stem phone area of interest
Cell Networks

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Peng et al. hESCs: H9 (WiCell, WA09) at passage 33 were passaged using collagenase IV equally onto Matrigel (BD, hESCqualified) and Matrix-B and cultured using defined medium mTeSR-1 (StemCell Technologies). Culture medium was changed daily and subculture was done every 5-7 days. Induced neural differentiation: The protocol for neural differentiation was adapted from WiCell Research Institute, National Stem Cell Bank. Karyotype: This was performed at KK Women’s and Children Hospital. B. Characterization of Matrices Decellularised matrices were immunofluorescently analysed with an array of antibodies (from Invitrogen, unless stated differently): mouse anti PDI, Alexa-Fluor 594 phalloidin, mouse anti collagen I (Sigma, C2456), rabbit anti fibronectin (Dako, A0245), mouse anti heparan sulfate (Seikagaku, 370255), mouse anti fibrillin (Chemicon, MAB 2499), rabbit anti decorin and rb anti biglycan (gift from Dr Larry Fisher), rb anti LTBP-1 (gift from Dr Carl Heldin, AB39).

The introductions of channels into a scaffold or hydrogel, or using mechanical loading to improve nutrient transfer, are two potential approaches to overcome this limitation. If both approaches are combined, the mechanical environment within dynamically compressed hydrogels will be modified by the introduction of microchannels into the construct. The objective of this study was to investigate how chondrocytes will respond to this altered mechanical environment. Isolated porcine chondrocytes were suspended in 2% agarose.

Ng et al. (9) also observed a heterogeneous development of material properties in free swelling chondrocyte-seeded agarose disks. It has been suggested that nutrient diffusion limitations are a possible reason for these heterogeneous constructs (10). Dynamic compression can enhance nutrient transport within agarose constructs (11). Altering the architecture of the constructs is another possible way to overcome nutrient transport limitations. Bian et al. (12) obtained a more homogeneous cartilaginous tissue upon the introduction of macroscopic channels throughout the depth of the construct.

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